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Thousands of endoparasitoid wasp species in the families Braconidae and Ichneumonidae harbor domesticated endogenous viruses (DEVs) in their genomes. This study focuses on ichneumonid DEVs, named ichnoviruses (IVs). Large quantities of DNA-containing IV virions are produced in ovary calyx cells during the pupal and adult stages of female wasps. Females parasitize host insects by injecting eggs and virions into the body cavity. After injection, virions rapidly infect host cells which is followed by expression of IV genes that promote the successful development of wasp offspring. IV genomes consist of two components: proviral segment loci that serve as templates for circular dsDNAs that are packaged into capsids, and genes from an ancestral virus that produce virions. In this study, we generated a chromosome-scale genome assembly forHyposoter didymatorthat harborsH. didymatorichnovirus (HdIV). We identified a total of 67 HdIV loci that are amplified in calyx cells during the wasp pupal stage. We then focused on an HdIV gene,U16, which is transcribed in calyx cells during the initial stages of replication. Sequence analysis indicated that U16 contains a conserved domain in primases from select other viruses. Knockdown ofU16by RNA interference inhibited virion morphogenesis in calyx cells. Genome-wide analysis indicatedU16knockdown also inhibited amplification of HdIV loci in calyx cells. Altogether, our results identified several previously unknown HdIV loci, demonstrated that all HdIV loci are amplified in calyx cells during the pupal stage, and showed that U16 is required for amplification and virion morphogenesis. 

Abstract. Geography and associated hydrological, hydroclimate and land-useconditions and their changes determine the states and dynamics of wetlandsand their ecosystem services. The influences of these controls are notlimited to just the local scale of each individual wetland but extend overlarger landscape areas that integrate multiple wetlands and their totalhydrological catchment – the wetlandscape. However, the data and knowledgeof conditions and changes over entire wetlandscapes are still scarce,limiting the capacity to accurately understand and manage critical wetlandecosystems and their services under global change. We present a newWetlandscape Change Information Database (WetCID), consisting of geographic,hydrological, hydroclimate and land-use information and data for 27wetlandscapes around the world. This combines survey-based local informationwith geographic shapefiles and gridded datasets of large-scale hydroclimateand land-use conditions and their changes over whole wetlandscapes.Temporally, WetCID contains 30-year time series of data for mean monthlyprecipitation and temperature and annual land-use conditions. Thesurvey-based site information includes local knowledge on the wetlands,hydrology, hydroclimate and land uses within each wetlandscape and on theavailability and accessibility of associated local data. This novel database(available through PANGAEA https://doi.org/10.1594/PANGAEA.907398; Ghajarniaet al., 2019) can support site assessments; cross-regional comparisons; andscenario analyses of the roles and impacts of land use, hydroclimatic andwetland conditions, and changes in whole-wetlandscape functions and ecosystemservices. 

Wetlands are often vital physical and social components of a country’s natural capital, as well as providers of ecosystem services to local and national communities. We performed a network analysis to prioritize Sustainable Development Goal (SDG) targets for sustainable development in iconic wetlands and wetlandscapes around the world. The analysis was based on the information and perceptions on 45 wetlandscapes worldwide by 49 wetland researchers of the Global Wetland Ecohydrological Network (GWEN). We identified three 2030 Agenda targets of high priority across the wetlandscapes needed to achieve sustainable development: Target 6.3—“Improve water quality”; 2.4—“Sustainable food production”; and 12.2—“Sustainable management of resources”. Moreover, we found specific feedback mechanisms and synergies between SDG targets in the context of wetlands. The most consistent reinforcing interactions were the influence of Target 12.2 on 8.4—“Efficient resource consumption”; and that of Target 6.3 on 12.2. The wetlandscapes could be differentiated in four bundles of distinctive priority SDG-targets: “Basic human needs”, “Sustainable tourism”, “Environmental impact in urban wetlands”, and “Improving and conserving environment”. In general, we find that the SDG groups, targets, and interactions stress that maintaining good water quality and a “wise use” of wetlandscapes are vital to attaining sustainable development within these sensitive ecosystems.